WESTERN BLOT HIV TEST FULL
WESTERN BLOT HIV TEST SERIES

Geenius HIV1/2 HIV Western blot HIV confirmatory tests.Ĭopyright © 2014 Elsevier B.V. The Bio-Rad Geenius HIV1/2 confirmatory system represents a reliable alternative to other confirmatory assays in HIV testing algorithms and provides clear improvement in quality management.

WESTERN BLOT HIV TEST FULL

In comparison with MP HIV BLOT 2.2, the Bio-Rad Geenius HIV1/2 assay was markedly time saving, allowed full traceability, automatic reading and interpretation. After excluding these last 13 samples, the specificity of Bio-Rad Geenius HIV1/2 assay reached 100%. Western blot misclassifies as HIV-1 >60 of HIV-2 infections.

WESTERN BLOT HIV TEST SERIES

If the test is positive, a series of bands are detected that indicate the specific binding of the persons antibody to specific HIV proteins. This presentation will also refer to individual HIV tests by brand. In this test, the HIV proteins are separated by size and electric charge, and the persons serum is layered on the test strip. If an ELISA test is negative, but you think you may have HIV, you should be tested.

If an ELISA test is positive, the Western blot test is usually administered to confirm the diagnosis. Two out of the eight indeterminate samples by MP HIV BLOT 2.2 that were confirmed negative after follow-up were tested indeterminate and one invalid, the other five were negative. This second test is called the Western blot test. The primary tests for diagnosing HIV and AIDs include: ELISA Test ELISA, which stands for enzyme-linked immunosorbent assay, is used to detect HIV infection. All five low-reactive samples by EIA, negative by HIV BLOT 2.2 were tested negative by Bio-Rad Geenius HIV1/2. All HIV negative serums by fourth-generation EIA were tested negative. After excluding early seroconversion samples, the sensitivity of Bio-Rad Geenius HIV1/2 assay reached 100%. Two out of the five HIV-2 reactive samples were tested positive HIV-2, two positive HIV-2 with HIV-1 cross-reaction and one HIV positive untypable. All HIV-1 non-B subtype samples were tested positive. Five out of 11 early seroconversion samples were tested positive, four negative and two indeterminate. The overall sensitivity for Bio-Rad Geenius HIV1/2 assay was 92%.

The samples were tested according to the manufacturer's guidelines. We have evaluated the recently Conformité Européenne (CE)-marked Bio-Rad Geenius human immunodeficiency virus (HIV)1/2 as a rapid and simple alternative to western blot for confirmation of HIV screening results.Ī total of 160 serum samples were tested: 44 HIV-1 reactive samples by a fourth-generation Murex HIV Ag/Ab and/or Vidas HIV Duo Ultra, five HIV-2 reactive samples, 15 HIV-1 non-B subtype samples and 11 confirmed HIV-1 early seroconversion samples, 72 nonreactive samples, eight indeterminate samples by MP HIV BLOT 2.2 confirmed negative after follow-up and five low-reactive samples by enzyme immunoassay (EIA) negative by MP HIV BLOT 2.2.

Western blot hiv test